Research Paper Volume 13, Issue 13 pp 16957—16973

The dual distinct role of telomerase in repression of senescence and myofibroblast differentiation

TERT binding to CDKN2A but not to ACTA2 promoter. Schematic illustration of ACTA2 (A) and CDKN2A (C) promoters are shown. PCR primers for ChIP assays are indicated by numbered bidirectional arrows, with their positions and lengths listed in the respective tables below the illustrations in (B) and (D). ChIP assays to assess TERT binding to ACTA2 (B) or CDKN2A (D) promoter in BJ5ta cells were performed using primers as numbered in (A) and (C), respectively. The cell DNA immunoprecipitated by YB-1 antibody was amplified by qPCR. One tenth of the supernatant before immunoprecipitation was used for the DNA input control. Results are expressed as % of input. N = 3 each group. *P ***P

Figure 4. TERT binding to CDKN2A but not to ACTA2 promoter. Schematic illustration of ACTA2 (A) and CDKN2A (C) promoters are shown. PCR primers for ChIP assays are indicated by numbered bidirectional arrows, with their positions and lengths listed in the respective tables below the illustrations in (B) and (D). ChIP assays to assess TERT binding to ACTA2 (B) or CDKN2A (D) promoter in BJ5ta cells were performed using primers as numbered in (A) and (C), respectively. The cell DNA immunoprecipitated by YB-1 antibody was amplified by qPCR. One tenth of the supernatant before immunoprecipitation was used for the DNA input control. Results are expressed as % of input. N = 3 each group. *P < 0.05. ***P < 0.001.