Figure 5. TERT enhanced YB-1 expression and phosphorylation was associated with repression of ACTA2 gene expression. (A) Expression of TERT, αSMA, p16, YB-1 and phosphorylated YB-1 (YB-1S102P) in whole cell lysates was analyzed by western blotting and the results of quantitative analysis shown as relative integration units (RIU) (B). β-actin or GAPDH was used as the internal control. ND, not detected. N = 3 each group in (B). (C) Control or YB-1 siRNA transfected BJ or BJ5ta cells were analyzed for expression of indicated proteins by western blotting, and the corresponding quantified protein levels are shown in the right panel. Representative blots from 3 separate experiments are shown. *P < 0.05. **P < 0.01.