Research Paper Volume 13, Issue 13 pp 17568—17591

Aging-dependent loss of GAP junction proteins Cx46 and Cx50 in the fiber cells of human and mouse lenses accounts for the diminished coupling conductance


Figure 9. Oxidative stress-induced changes of the connexins Cx43, Cx46 and Cx50 in the whole lenses of 4-week mice after treatment by 10 milli-units (mU) of glucose oxidase (GO) for different time. Treatment of mouse lenses by 10 mU GO caused in vitro cataractogenesis in 24 and 48 hours (data not shown). (A) Western blot analysis of Cx46 and Cx50 in mouse lenses after treatment with 10 mU glucose oxidase for 0–48 h as indicated. α-Actinin was showed as a loading control. (B) Quantification of the western blot results in (A). NS, not significant, *p < 0.05, **p < 0.01, ***p < 0.001.