Research Paper Volume 13, Issue 13 pp 17673—17689

Circular RNA circCCDC66 promotes glioma proliferation by acting as a ceRNA for miR-320a to regulate FOXM1 expression

CircCCDC66 could regulate FOXM1 expression by sponging miR-320a. (A) The links between circCCDC66 and miR-320a. (B) Luciferase reporter assay was conducted to evaluate the interaction ability between miR-320a and circCCDC66. (C) Relative FOXM1 mRNA expression in glioma cells with miR-320a mimics. (D) Cells with miR-320a inhibitor had higher expression levels of FOXM1 mRNA. (E) Luciferase reporter assay was conducted to evaluate the interaction ability between miR-320a and FOXM1 3’-UTR. (F, G) MiR-320a inhibitor can resist the decreased FOXM1 mRNA expression caused by si-circCCDC66. *P P

Figure 5. CircCCDC66 could regulate FOXM1 expression by sponging miR-320a. (A) The links between circCCDC66 and miR-320a. (B) Luciferase reporter assay was conducted to evaluate the interaction ability between miR-320a and circCCDC66. (C) Relative FOXM1 mRNA expression in glioma cells with miR-320a mimics. (D) Cells with miR-320a inhibitor had higher expression levels of FOXM1 mRNA. (E) Luciferase reporter assay was conducted to evaluate the interaction ability between miR-320a and FOXM1 3’-UTR. (F, G) MiR-320a inhibitor can resist the decreased FOXM1 mRNA expression caused by si-circCCDC66. *P < 0.05, **P < 0.01.