Research Paper Volume 13, Issue 13 pp 17690—17706

α-Cyperone (CYP) down-regulates NF-κB and MAPKs signaling, attenuating inflammation and extracellular matrix degradation in chondrocytes, to ameliorate osteoarthritis in mice

Effects of CYP on survival rate of chondrocytes. (A) Structural chemical formula of CYP. (B, C) CYP’s cytotoxicity of in an increasing concentration (0, 0.75, 1.5, 3, 6 μM) on chondrocytes as measured by CCK-8 assay after a 24 or 48-hour incubation. (D) Morphology of chondrocytes as observed under a microscope following treatment by IL-1β (10 ng/ml) combined with or without CYP (3 μM) after 24 hours of incubation (scale bar: 150 μm). Data presented are means ± S.D. of five independent experiments. * p p

Figure 1. Effects of CYP on survival rate of chondrocytes. (A) Structural chemical formula of CYP. (B, C) CYP’s cytotoxicity of in an increasing concentration (0, 0.75, 1.5, 3, 6 μM) on chondrocytes as measured by CCK-8 assay after a 24 or 48-hour incubation. (D) Morphology of chondrocytes as observed under a microscope following treatment by IL-1β (10 ng/ml) combined with or without CYP (3 μM) after 24 hours of incubation (scale bar: 150 μm). Data presented are means ± S.D. of five independent experiments. * p < 0.05 and ** p < 0.01 vs. the control group, n=5.