Figure 2. CYP inhibits inflammation in chondrocytes induced by IL-1β (10 ng/ml). Expression of INOS, COX-2, TNF-α and IL-6 genes were determined by real-time PCR (A, B). Levels of INOS, COX-2, TNF-α and IL-6 inflammatory mediators in rat chondrocytes were detected by western blot assay (C) and quantified by Image Lab software (D, E). Levels of PGE2 and Nitrite as measured by ELISA and Griess reagent method (F). Data are presented as means ± S.D. ## means p < 0.01 vs. the control group and ** p < 0.01, * p < 0.05 vs. the IL-1β alone group, n=5.