Figure 3. Effects of CYP on IL-1β induced ECM degradation in chondrocytes. Levels of type II collagen, aggrecan, MMP-13 and ADAMTS5 were measured by ELISA (A). The type II collagen was assayed through cell immunofluorescence combined with DAPI staining for nuclei (scale bar:100 μm) (B). Expressions levels for type II collagen, ADAMTS5, MMP-13 and MMP-3 were detected by western blot assay (C) and quantified by Image Lab software (D). Data are presented as means ± S.D.## means p < 0.01 vs. the control group and ** p < 0.01, * p < 0.05 vs. the IL-1β alone group, n=5.