Research Paper Volume 13, Issue 13 pp 17690—17706

α-Cyperone (CYP) down-regulates NF-κB and MAPKs signaling, attenuating inflammation and extracellular matrix degradation in chondrocytes, to ameliorate osteoarthritis in mice

Effect of CYP on MAPK activation induced by IL-1β (10 ng/ml). (A) Molecular docking results of CYP with p38, JNK and ERK showed that CYP was embedded in the binding pockets of proteins. Hydrogen bonds were built between CYP and LYS-53, ASP-168, GLU-109, MET-111, LEU-107 and MET-108. Expression levels of p-p38, p38, p-ERK, ERK, p-JNK and JNK were detected by western blot assay (B) and quantified by Image Lab software (C). Data presented are means ± S.D. ## means p vs. the control group and ** p p vs. the IL-1β alone group, n=5.

Figure 5. Effect of CYP on MAPK activation induced by IL-1β (10 ng/ml). (A) Molecular docking results of CYP with p38, JNK and ERK showed that CYP was embedded in the binding pockets of proteins. Hydrogen bonds were built between CYP and LYS-53, ASP-168, GLU-109, MET-111, LEU-107 and MET-108. Expression levels of p-p38, p38, p-ERK, ERK, p-JNK and JNK were detected by western blot assay (B) and quantified by Image Lab software (C). Data presented are means ± S.D. ## means p < 0.01 vs. the control group and ** p < 0.01, * p < 0.05 vs. the IL-1β alone group, n=5.