Research Paper Volume 13, Issue 17 pp 21090—21101

Effect of the Notch1-mediated PI3K-Akt-mTOR pathway in human osteosarcoma

Notch1 induced S phase cycle arrest in human osteosarcoma cells. (A) Notch1 induced by doxycycline in different time induced S phase cell cycle arrest in human osteosarcoma cells. Cells were treated with doxycycline for 0, 12, 24, 36, 48 and 60 h and analyzed by flow cytometry. The percentage of cell population at G1, S, and G2/M phases were represented as mean ± SD of three independent experiments. (B) The histogram of percentage of S phase in different groups. *P

Figure 2. Notch1 induced S phase cycle arrest in human osteosarcoma cells. (A) Notch1 induced by doxycycline in different time induced S phase cell cycle arrest in human osteosarcoma cells. Cells were treated with doxycycline for 0, 12, 24, 36, 48 and 60 h and analyzed by flow cytometry. The percentage of cell population at G1, S, and G2/M phases were represented as mean ± SD of three independent experiments. (B) The histogram of percentage of S phase in different groups. *P<0.05, significantly different compared with the untreated control group. All the experiments were conducted in triplicates.