Figure 3. Notch1 induced osteosarcoma cell apoptosis. (A) Notch1 induced by doxycycline in different time induced osteosarcoma cell apoptosis. Cells were treated with doxycycline for 0, 12, 24, 36, 48 and 60 h and analyzed using Annexin V-FITC/PI flow cytometry. (B) The histogram indicated that apoptosis proportion from three separate experiments in different groups. *P<0.05, significantly different compared with the untreated control group. All the experiments were conducted in triplicates.