Research Paper Volume 13, Issue 14 pp 18257—18273

The lncRNA H19/miR-541-3p/Wnt/β-catenin axis plays a vital role in melatonin-mediated osteogenic differentiation of bone marrow mesenchymal stem cells

miR-541-3p contained the binding sites of H19 and APN mRNA. (A) The binding sites between miR-541-3p, H19 and APN mRNA were shown. (B) BMSCs were transfected with miR-541-3p or miR-NC, then the RIP experiment was performed to explore the correlation between miR-541-3p and H19, miR-541-3p and APN mRNA. The enrichment of H19, miR-541-3p and APN mRNA were determined by qRT-PCR. NS P>0.05, # PPC) BMSCs was transfected with WT-H19/MUT-H19 or WT-APN/MUT-APN and miR-541-3p or miR-NC. The dual-luciferase reporter assay was implemented to verify the association between miR-541-3p and H19 and APN mRNA. NS P>0.05, **P

Figure 6. miR-541-3p contained the binding sites of H19 and APN mRNA. (A) The binding sites between miR-541-3p, H19 and APN mRNA were shown. (B) BMSCs were transfected with miR-541-3p or miR-NC, then the RIP experiment was performed to explore the correlation between miR-541-3p and H19, miR-541-3p and APN mRNA. The enrichment of H19, miR-541-3p and APN mRNA were determined by qRT-PCR. NS P>0.05, # P<0.05 (vs. miR-NC group), ***P<0.001(vs. IgG group). (C) BMSCs was transfected with WT-H19/MUT-H19 or WT-APN/MUT-APN and miR-541-3p or miR-NC. The dual-luciferase reporter assay was implemented to verify the association between miR-541-3p and H19 and APN mRNA. NS P>0.05, **P<0.01 (vs. miR-NC group). Data were presented as mean ±SEM (n =3) and analyzed using one-way analysis of variance.