Research Paper Volume 13, Issue 15 pp 19306—19316

Figure 2. Deletion of HAGLR inhibited the viability, proliferation, migration and invasion of BT549 cells. Small interfering RNA of HAGLR (si-HAGLR) and its Scramble were transfected into BT549 cells. (A) The knockdown efficiency of si- HAGLR was determined by qRT-PCR. (B) CCK8 assay was used to test viability of BT549 cells. (C) EdU assay was to detected proliferation of BT549 cells. Scale bar, 100 μm. (D) Wound healing assay was to evaluate migration of BT549 cells. Scale bar, 100 μm. (E) Transwell assay was to examine invasion of BT549 cells. Scale bar, 50 μm. Data are mean ± SD; ^*P < 0.05, ^**P < 0.01.