Research Paper Volume 13, Issue 14 pp 18340—18359

CircSPIDR acts as a tumour suppressor in cervical adenocarcinoma by sponging miR-431-5p and regulating SORCS1 and CUBN expression

CircSPIDR inhibits cell growth and promotes apoptosis in CADC cells in vitro. (A, C) CCK-8 assays demonstrated that circSPIDR overexpression reduced HeLa cell viability (A), while circSPIDR inhibition with specific siRNAs (si-circSPIDR#1 or si-circSPIDR#2) increased HeLa cell viability (C). (B, D) Colony formation assays indicated that circSPIDR overexpression suppressed colony formation in HeLa cells (B), while circSPIDR inhibition promoted colony formation (D). (E, F) Representative images from flow cytometry analysis of apoptosis. Apoptotic cells were determined using Annexin V-FITC (X-axis) and PI (Y-axis) staining. CircSPIDR overexpression promoted HeLa cell apoptosis, while circSPIDR inhibition had the opposite effect. *P **P ***P

Figure 2. CircSPIDR inhibits cell growth and promotes apoptosis in CADC cells in vitro. (A, C) CCK-8 assays demonstrated that circSPIDR overexpression reduced HeLa cell viability (A), while circSPIDR inhibition with specific siRNAs (si-circSPIDR#1 or si-circSPIDR#2) increased HeLa cell viability (C). (B, D) Colony formation assays indicated that circSPIDR overexpression suppressed colony formation in HeLa cells (B), while circSPIDR inhibition promoted colony formation (D). (E, F) Representative images from flow cytometry analysis of apoptosis. Apoptotic cells were determined using Annexin V-FITC (X-axis) and PI (Y-axis) staining. CircSPIDR overexpression promoted HeLa cell apoptosis, while circSPIDR inhibition had the opposite effect. *P < 0.05; **P < 0.01; ***P < 0.001.