Research Paper Volume 13, Issue 13 pp 17830—17846

Linc00941 regulates esophageal squamous cell carcinoma via functioning as a competing endogenous RNA for miR-877-3p to modulate PMEPA1 expression


Figure 2. Knockdown of linc00941 suppressed ESCC cell proliferation, invasion and migration. (A) Linc00941 was up-regulated in ESCC cell lines as determined by qRT-PCR. (B, C) Linc00941 siRNA transfection attenuated the clonogenicity ability of KYSE-510 and KYSE-30 cells. (D, E) Knockdown of linc00941 suppressed the proliferative ability of KYSE-510 and KYSE-30 cells as determined by CCK-8 assay. (F, G) Knockdown of linc00941 attenuated the KYSE-510 and KYSE-30 cell proliferation as determined by EdU assay. (H, I) Linc00941 siRNA transfection reduced the number of invaded KYSE-510 and KYSE-30 cells as determined by Transwell invasion assay. (J, K) Linc00941 siRNA transfection retarded wound closure of KYSE-510 and KYSE-30 cells as determined by Wound healing assay. (L, M) Linc00941 knockdown suppressed the tumor growth of the KYSE-510 and KYSE-30 cells. (N, O) The tumor weight dissected from the nude mice in different groups was determined. N = 3-6. *P<0.05, **P<0.01 and ***P<0.001 compared between different treatment groups.