**Figure 2.** **7-day bleomycin protocol disrupts mitochondrial homeostasis in alveolar epithelial cells.** (**A**, **B**) Mitochondrial oxygen consumption in control and chronically injured MLE12 cells. Right, bar graph depicting basal oxygen consumption rate (OCR), ATP production and spare respiratory capacity. (**C**) mtDNA damage quantification in control and bleomycin injured cells. (**D**) Quantification of reactive oxygen species (ROS) by MitoSox staining in control and chronically injured MLE12 cells. Seahorse data are representative of 3 separate experiments. Statistical significance was assessed by unpaired Student’s t-test except for Seahorse analyses in which two-way analysis of variance (ANOVA) followed by Sidak post hoc analysis was used to adjust for multiple comparisons. Statistical significance was achieved when P < 0.05 at 95% confidence interval. Given multiple * p< 0.05, **P < 0.01, ***P < 0.001 versus control group, n=6.