Figure 2. 7-day bleomycin protocol disrupts mitochondrial homeostasis in alveolar epithelial cells. (A, B) Mitochondrial oxygen consumption in control and chronically injured MLE12 cells. Right, bar graph depicting basal oxygen consumption rate (OCR), ATP production and spare respiratory capacity. (C) mtDNA damage quantification in control and bleomycin injured cells. (D) Quantification of reactive oxygen species (ROS) by MitoSox staining in control and chronically injured MLE12 cells. Seahorse data are representative of 3 separate experiments. Statistical significance was assessed by unpaired Student’s t-test except for Seahorse analyses in which two-way analysis of variance (ANOVA) followed by Sidak post hoc analysis was used to adjust for multiple comparisons. Statistical significance was achieved when P < 0.05 at 95% confidence interval. Given multiple * p< 0.05, **P < 0.01, ***P < 0.001 versus control group, n=6.