Research Paper Volume 13, Issue 13 pp 16922—16937

A model of the aged lung epithelium in idiopathic pulmonary fibrosis

The HSF1 activity is impaired in MLE12 cells after our 7-day injury protocol. (A) Transcript levels for Hsp90, Hsp70, and Hsp40 in control and bleomycin injured MLE12 cells. (B) WB for HSF1, p-HSF1ser307, p-HSF1ser326 in control, and bleomycin injured MLE12 cells (with Laminin-B1 loading control). Densitometry is shown on the right. Results of densitometry analysis are depicted in bar graphs (n= 6, per group). Statistical significance was assessed by unpaired Student’s t-test for two groups. * p

Figure 7. The HSF1 activity is impaired in MLE12 cells after our 7-day injury protocol. (A) Transcript levels for Hsp90, Hsp70, and Hsp40 in control and bleomycin injured MLE12 cells. (B) WB for HSF1, p-HSF1ser307, p-HSF1ser326 in control, and bleomycin injured MLE12 cells (with Laminin-B1 loading control). Densitometry is shown on the right. Results of densitometry analysis are depicted in bar graphs (n= 6, per group). Statistical significance was assessed by unpaired Student’s t-test for two groups. * p< 0.05, **P < 0.01, ***P < 0.001 versus control group, n=6.