Research Paper Volume 13, Issue 15 pp 19317—19338

The deubiquitinase OTUD3 stabilizes ACTN4 to drive growth and metastasis of hepatocellular carcinoma

ACTN4 is critical for OTUD3-mediated HCC cells progression in vitro and in vivo. (A) Western blot confirming the downregulation of OTUD3 abated increased ACTN4 expression in HCCLM3 cells. (B, C) EdU assay evaluating the effect of OTUD3 knockdown on accelerated HCC cell growth enhanced by ACTN4 upregulation. (D) Quantification of HCC cell transwell migration and invasion results.

Figure 6. ACTN4 is critical for OTUD3-mediated HCC cells progression in vitro and in vivo. (A) Western blot confirming the downregulation of OTUD3 abated increased ACTN4 expression in HCCLM3 cells. (B, C) EdU assay evaluating the effect of OTUD3 knockdown on accelerated HCC cell growth enhanced by ACTN4 upregulation. (D) Quantification of HCC cell transwell migration and invasion results.