Figure 2. CAFs expression of GAL-1/ LGALS1 promotes the invasion and metastasis ability of GC cell lines in vitro. (A, B) GAL-1 protein and LGALS1 mRNA levels in CAFs and GC cell lines, GAL-1/LGALS1 levels were high in CAFs. (C, D) Treatment of MGC-803 and SGC-7901 cells with CM-CAFs significantly increased GAL-1 protein levels and LGALS1 mRNA expression. (E) CM-CAFs increased the proliferation of MGC-803 and SGC-7901 cells, and the proliferation effect was abolished when the medium contained 10 μg/mL mitomycin C. (F, G) MGC-803 and SGC-7901 cells treated with CM-CAFs exhibited a significantly enhanced migration capacity compared with the wild-type control (P < 0.01). Magnification: ×40. (H, I) Transwell assay showing that MGC-803 and SGC-7901 cells treated with CM-CAFs had increased cell invasion and migration abilities (P < 0.01). Magnification: ×200.