Research Paper Volume 13, Issue 13 pp 17097—17117

Inhibition of heat shock proteins increases autophagosome formation, and reduces the expression of APP, Tau, SOD1 G93A and TDP-43

Neratinib and AR12 interact to reduce the expression of Tau and Tau 301L in Vero cells. (A, B) Vero cells were transfected with plasmids to express Tau-GFP or Tau 301L-GFP. After 24h, cells were treated for 6h or 12h with vehicle control, AR12 (2 μM), neratinib (50 nM) or the drugs in combination. Cells were fixed in place and the detection of Tau levels determined using either GFP tag fluorescence or by in cell immunostaining (n = 3 +/-SD) * p

Figure 7. Neratinib and AR12 interact to reduce the expression of Tau and Tau 301L in Vero cells. (A, B) Vero cells were transfected with plasmids to express Tau-GFP or Tau 301L-GFP. After 24h, cells were treated for 6h or 12h with vehicle control, AR12 (2 μM), neratinib (50 nM) or the drugs in combination. Cells were fixed in place and the detection of Tau levels determined using either GFP tag fluorescence or by in cell immunostaining (n = 3 +/-SD) * p < 0.05 less than vehicle control; ** p < 0.05 less than neratinib single agent value.