Research Paper Advance Articles

Agomelatine prevents angiotensin II-induced endothelial and mononuclear cell adhesion

Overexpression of Egr-1 abolished the protective effect of agomelatine against Ang II in HUVECs. HUVECs were transfected with Egr-1 plasmid, followed by stimulation with Ang II (1 μM) with or without agomelatine (20 μM) for 24 h. (A) Successful overexpression of Egr-1; (B) Protein of VCAM-1 and ICAM-1; (C) Calcein-AM staining method was used to measure attachment of THP-1 cells to HUVECs (##, **, &&, P

Figure 7. Overexpression of Egr-1 abolished the protective effect of agomelatine against Ang II in HUVECs. HUVECs were transfected with Egr-1 plasmid, followed by stimulation with Ang II (1 μM) with or without agomelatine (20 μM) for 24 h. (A) Successful overexpression of Egr-1; (B) Protein of VCAM-1 and ICAM-1; (C) Calcein-AM staining method was used to measure attachment of THP-1 cells to HUVECs (##, **, &&, P<0.01 vs. vehicle control, Ang II, Ang II+ 20 μM agomelatine group).