Research Paper Volume 13, Issue 14 pp 18527—18544

LncRNA CCAT1 promotes prostate cancer cells proliferation, migration, and invasion through regulation of miR-490-3p/FRAT1 axis

miR-490-3p is directly targeted by CCAT1. (A) Heatmap of the 14 differentially expressed miRNAs between PCa tissues and adjacent normal tissues in GSE60117. (B) miR-490-3p was both down-regulated in PCa and targeted by CCAT1. (C) Binding sites of CCAT1 and miR-490-3p. (D) Dual-luciferase reporter assay detection of the interaction between CCAT1 and miR-490-3p in LnCaP and PC3 cells. (E) miR-490-3p expression in PCa tissues and adjacent normal tissues of 10 clinical samples. miR-490-3p was decreased in PCa cells compared to that in the normal prostate tissues. (F) Correlation analysis of CCAT1 expression and miR-490-3p expression in PCa tissues. miR-490-3p expression was negatively correlated with CCAT1 level. (G) miR-490-3p expression in RWPE-1, LnCaP, and PC3 cells. miR-490-3p expression was suppressed in PCa cell lines. *P P

Figure 4. miR-490-3p is directly targeted by CCAT1. (A) Heatmap of the 14 differentially expressed miRNAs between PCa tissues and adjacent normal tissues in GSE60117. (B) miR-490-3p was both down-regulated in PCa and targeted by CCAT1. (C) Binding sites of CCAT1 and miR-490-3p. (D) Dual-luciferase reporter assay detection of the interaction between CCAT1 and miR-490-3p in LnCaP and PC3 cells. (E) miR-490-3p expression in PCa tissues and adjacent normal tissues of 10 clinical samples. miR-490-3p was decreased in PCa cells compared to that in the normal prostate tissues. (F) Correlation analysis of CCAT1 expression and miR-490-3p expression in PCa tissues. miR-490-3p expression was negatively correlated with CCAT1 level. (G) miR-490-3p expression in RWPE-1, LnCaP, and PC3 cells. miR-490-3p expression was suppressed in PCa cell lines. *P < 0.05, **P < 0.01, compared with NC mimics group or RWPE-1 group.