Research Paper Volume 13, Issue 14 pp 18527—18544

LncRNA CCAT1 promotes prostate cancer cells proliferation, migration, and invasion through regulation of miR-490-3p/FRAT1 axis

FRAT1 was directly targeted by miR-490-3p. (A) 7 potential target genes of miR-490-3p were screened out. (B) qRT-PCR detection of the 7 potential target genes in PCa tissues. Among them, HMGA2, SAMD5, FRAT1, and MYO6 were greatly up-regulated in PCa tissues compared with adjacent normal tissues. (C, D) Knockdown of CCAT1 greatly down-regulated the expression of HMGA2, SAMD5, and FRAT1. (E) Predicted binding sites between miR-490-3p and FRAT1. (F, G) FRAT1 expression was positively correlated with CCAT1 level and negatively correlated with miR-490-3p level. (H) Dual-luciferase reporter assay detection of the interaction between miR-490-3p and FRAT1 in LnCaP and PC3 cells. (I, J) mRNA and protein expression of FRAT1 in RWPE-1, LnCaP, and PC3 cells. Both FRAT1 mRNA and protein expression were up-regulated in PCa cell lines. (K, L) miR-490-3p overexpression greatly down-regulated the mRNA and protein expression of FRAT1, while down-regulation of miR-490-3p promoted the mRNA and protein expression of FRAT1. *P P

Figure 7. FRAT1 was directly targeted by miR-490-3p. (A) 7 potential target genes of miR-490-3p were screened out. (B) qRT-PCR detection of the 7 potential target genes in PCa tissues. Among them, HMGA2, SAMD5, FRAT1, and MYO6 were greatly up-regulated in PCa tissues compared with adjacent normal tissues. (C, D) Knockdown of CCAT1 greatly down-regulated the expression of HMGA2, SAMD5, and FRAT1. (E) Predicted binding sites between miR-490-3p and FRAT1. (F, G) FRAT1 expression was positively correlated with CCAT1 level and negatively correlated with miR-490-3p level. (H) Dual-luciferase reporter assay detection of the interaction between miR-490-3p and FRAT1 in LnCaP and PC3 cells. (I, J) mRNA and protein expression of FRAT1 in RWPE-1, LnCaP, and PC3 cells. Both FRAT1 mRNA and protein expression were up-regulated in PCa cell lines. (K, L) miR-490-3p overexpression greatly down-regulated the mRNA and protein expression of FRAT1, while down-regulation of miR-490-3p promoted the mRNA and protein expression of FRAT1. *P < 0.05, **P < 0.01, compared with the normal group or the si-NC group or the RWPE-1 group.