Research Paper Volume 13, Issue 14 pp 18527—18544

LncRNA CCAT1 promotes prostate cancer cells proliferation, migration, and invasion through regulation of miR-490-3p/FRAT1 axis

Suppression of FRAT1 inhibited cell proliferation, migration, and invasion in PCa cells. (A) Expression of FRAT1 in LnCaP and PC3 cells transfected with si-FRAT1 or pcDNA-FRAT1. (B) Cell viability of LnCaP and PC3 cells. Cell viability was suppressed in PCa cells treated with si-FRAT1 and promoted in PCa cells with pcDNA-FRAT1. (C) EdU staining results in LnCaP and PC3 cells. si-FRAT1 treatment suppressed cell proliferation while pcDNA-FRAT1 treatment promoted cell proliferation in PCa cells. Scale bar: 50 μm. (D) Cell apoptosis detection of LnCaP and PC3 cells by flow cytometry detection. PCa cells with lower FRAT1 expression had higher apoptosis rate, while PCa cells with higher FRAT1 expression had lower apoptosis rate. (E, F) Cell migration and invasion of LnCaP and PC3 cells in Transwell assays. Down-regulation of FRAT1 inhibited PCa cells migration and invasion, and overexpression of FRAT1 promoted PCa cells migration and invasion. (G) Western blot of EMT-associated protein in each group of PCa cells. E-cadherin expression was up-regulated while N-cadherin and Vimentin expression was suppressed in PCa cells transfected with si-FRAT1. PCa cells transfected with pcDNA-FRAT1 showed the opposite results. *P P P P

Figure 8. Suppression of FRAT1 inhibited cell proliferation, migration, and invasion in PCa cells. (A) Expression of FRAT1 in LnCaP and PC3 cells transfected with si-FRAT1 or pcDNA-FRAT1. (B) Cell viability of LnCaP and PC3 cells. Cell viability was suppressed in PCa cells treated with si-FRAT1 and promoted in PCa cells with pcDNA-FRAT1. (C) EdU staining results in LnCaP and PC3 cells. si-FRAT1 treatment suppressed cell proliferation while pcDNA-FRAT1 treatment promoted cell proliferation in PCa cells. Scale bar: 50 μm. (D) Cell apoptosis detection of LnCaP and PC3 cells by flow cytometry detection. PCa cells with lower FRAT1 expression had higher apoptosis rate, while PCa cells with higher FRAT1 expression had lower apoptosis rate. (E, F) Cell migration and invasion of LnCaP and PC3 cells in Transwell assays. Down-regulation of FRAT1 inhibited PCa cells migration and invasion, and overexpression of FRAT1 promoted PCa cells migration and invasion. (G) Western blot of EMT-associated protein in each group of PCa cells. E-cadherin expression was up-regulated while N-cadherin and Vimentin expression was suppressed in PCa cells transfected with si-FRAT1. PCa cells transfected with pcDNA-FRAT1 showed the opposite results. *P < 0.05, **P < 0.01, compared with the si-NC group; #P < 0.05, ##P < 0.01, compared with the empty vector group.