Research Paper Volume 13, Issue 14 pp 18586—18605

Circular RNA circSP3 promotes hepatocellular carcinoma growth by sponging microRNA-198 and upregulating cyclin-dependent kinase 4

Identification of circSP3 in HCC cells and confirmation of its circular structure. (A) The predicted binding between circSP3 and miR-198. (B) Schematic illustration demonstrating that circSP3 is located on chromosome 2q31.1 and cyclized from exons of SP3. (C) The PCR products of circSP3 were confirmed through Sanger sequencing. (D) CircSP3 was detected in Huh-7 cells. As expected, divergent primers detected circSP3 in cDNA but not in gDNA. GAPDH was used as a negative control. (E) The relative levels of circSP3 and SP3 were assessed through qRT-PCR analysis in cells treated with or without RNase R. *p

Figure 1. Identification of circSP3 in HCC cells and confirmation of its circular structure. (A) The predicted binding between circSP3 and miR-198. (B) Schematic illustration demonstrating that circSP3 is located on chromosome 2q31.1 and cyclized from exons of SP3. (C) The PCR products of circSP3 were confirmed through Sanger sequencing. (D) CircSP3 was detected in Huh-7 cells. As expected, divergent primers detected circSP3 in cDNA but not in gDNA. GAPDH was used as a negative control. (E) The relative levels of circSP3 and SP3 were assessed through qRT-PCR analysis in cells treated with or without RNase R. *p<0.05, **p<0.01 and ***p<0.001.