Research Paper Volume 13, Issue 14 pp 18586—18605

Circular RNA circSP3 promotes hepatocellular carcinoma growth by sponging microRNA-198 and upregulating cyclin-dependent kinase 4

CircSP3 induces HCC cell proliferation, migration and invasion by sponging miR-198 and upregulating CDK4. CircSP3-overexpressing Hep-3B cells were infected with miR-198 mimics or miR-198 mimic controls, and circSP3-knockdown Huh-7 cells were infected with miR-198 inhibitors or miR-198 inhibitor controls. (A, B) CCK-8, (C, D) Transwell migration and (E, F) Transwell invasion assays were conducted to assess cell proliferation, migration and invasion, respectively. (G, H) The effects of circSP3 and miR-198 expression on CDK4 expression were quantified using Western blotting. *p

Figure 7. CircSP3 induces HCC cell proliferation, migration and invasion by sponging miR-198 and upregulating CDK4. CircSP3-overexpressing Hep-3B cells were infected with miR-198 mimics or miR-198 mimic controls, and circSP3-knockdown Huh-7 cells were infected with miR-198 inhibitors or miR-198 inhibitor controls. (A, B) CCK-8, (C, D) Transwell migration and (E, F) Transwell invasion assays were conducted to assess cell proliferation, migration and invasion, respectively. (G, H) The effects of circSP3 and miR-198 expression on CDK4 expression were quantified using Western blotting. *p<0.05, **p<0.01 and ***p<0.001.