Research Paper Volume 13, Issue 14 pp 18586—18605

Circular RNA circSP3 promotes hepatocellular carcinoma growth by sponging microRNA-198 and upregulating cyclin-dependent kinase 4

Silencing of hsa

Figure 9. Silencing of hsa_circSP3 inhibits HCC formation in vivo. (A, B) The tumor sizes were recorded on day 32. (C, D) The rate of tumor growth was calculated every 4 days for 32 days. (E, F) The tumor weights were recorded on day 32. (G, H) The relative levels of circSP3 and miR-198 in tumors were detected using qRT-PCR. (I) CDK4 expression was quantified using Western blotting. (J) Immunohistochemistry revealed that Ki-67 levels were significantly lower in the LV3-circSP3 group than in the LV3-NC group. *p<0.05, **p<0.01 and ***p<0.001.