Figure 1. Reduced expression of HDACs expression in replicative senescence. (A, B) Representative Western blots showing HDACs 1-7 and p16INK-4A protein level in young (early passage, Y), intermediate (I) or replicative senescent (RS) AG04431 cells (A) or primary normal human dermal fibroblasts (NHDFs) isolated from an adult donor (57 years) or a young donor (3 years) (B). (C, D) Quantifications of the protein level of HDACs 1-7 and p16 INK-4A, with α-tubulin (AG04431, n=4) or GAPDH (NHDFs, n=2) as loading control. (E, F) Class IIa HDACs activity in AG04431 (e) (n=3) or in NHDFs (F) (n=3). Statistical analyses were performed using a t-test (*: p<0.05).