Figure 3. Isolation and identification of EMPs and EMPs mediated platelet activation. (A) The positive rate of CD144 in the plasma before and after isolation. (B) Transmission electron microscopy was used to observe the obtained microparticles with a diameter >100 nm. (C) Quantification and size estimation of EMPs by qNANO. (D) Adenosine diphosphate (ADP) (10 μg/ml), EMPs (2×107/ml), and an equal amount of PBS were used to stimulate the platelets of healthy people. Detect platelet expression of CD62p by flow cytometry. Histograms represent the CD62p% change. **P<0.01 vs. control, ###P<0.001 vs. EMPs treatment (n=4-11). Data are analyzed by one-way ANOVA.