Research Paper Volume 13, Issue 14 pp 18718—18739

Endothelial microparticle-associated protein disulfide isomerase increases platelet activation in diabetic coronary heart disease

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Figure 3. Isolation and identification of EMPs and EMPs mediated platelet activation. (A) The positive rate of CD144 in the plasma before and after isolation. (B) Transmission electron microscopy was used to observe the obtained microparticles with a diameter >100 nm. (C) Quantification and size estimation of EMPs by qNANO. (D) Adenosine diphosphate (ADP) (10 μg/ml), EMPs (2×107/ml), and an equal amount of PBS were used to stimulate the platelets of healthy people. Detect platelet expression of CD62p by flow cytometry. Histograms represent the CD62p% change. **P<0.01 vs. control, ###P<0.001 vs. EMPs treatment (n=4-11). Data are analyzed by one-way ANOVA.