Figure 4. GAS6 restores the Gas6 silencing- and/or maternal age-induced decline in fertility. (A) Microphotographs of PN embryos after in vitro fertilization. Aged oocytes were treated without (control) or with the GAS6 protein (GAS6-rescued) and then fertilized after in vitro culture. White triangles indicate PN formation. Scale bars represent 100 μm. (B) Percentage of oocytes showing PN formation after in vitro fertilization. Experiments were repeated at least three times, and data are presented as the means ± SEM. Different letters indicate significant differences at p<0.05.