Research Paper Volume 13, Issue 15 pp 19375—19396

THBS4/integrin α2 axis mediates BM-MSCs to promote angiogenesis in gastric cancer associated with chronic Helicobacter pylori infection


Figure 1. BM-MSCs promote tumorigenicity and angiogenesis in H. pylori-induced GC. (A) In BMT mice, engraftment of LSL-tdTomato marrow-derived cells in mouse gastric tissues was tracked with tdTomato staining. n= 4. (B) Representative gastric mucosa histopathology of gastric intraepithelial neoplasia (GIN) in BMT mice. (C) Quantification of GIN in B. (D) IF of GFP in H. pylori-infected stomachs transplanted with GFP-labeled BM-MSCs. (E) Representative gastric mucosa histopathology in H. pylori-infected stomachs with (H. pylori+ MSC) or without (H. pylori) BM-MSC transplantation. n= 30 mice in each group. (F) Quantification of dysplasia and GC in D. (G) IHC of CD31 and NG2 in H. pylori or H. pylori+ MSC group. (H) Quantification of F. Multiple fields (at least six) from three different mice were analyzed. (I) IF of CD31 and NG2 in H. pylori or H. pylori+ MSC group. Cell nuclei, DAPI; CD31, Cy3; NG2, Cy3. HP: H. pylori. MSC: bone marrow-derived mesenchymal stem cells. IF: immunofluorescence. The results are shown as the mean ± SD. *, P < 0.05; **, P < 0.01.