Figure 3. BM-MSC engraftment leads to THBS4 overexpression during H. pylori infection. (A) Volcano plot presentation of differentially expressed genes of gastric tissues in H. pylori versus H. pylori+ MSCs mice. (B) Enrichment analysis of differential genes. (C) RT-PCR verified the expression of differential genes associated with angiogenesis. THBS4 was the most highly expressed gene. n= 10 mice in each group. (D, E) Western blot analysis showed that BM-MSC transplantation led to THBS4 overexpression. n= 3 mice in each group. (F, G) IHC (F) and IF (G) of THBS4 in H. pylori+ MSCs or H. pylori mice. n= 3 mice per group Cell nuclei, DAPI. THBS4, FITC. Magnification: × 200 and × 400. (H) Quantification of F. (I, J) Western blot analysis of THBS4 revealed that H. pylori pretreatment increased the protein expression of THBS4 in BM-MSCs. n= 3 wells per group.