Figure 2. CF was increased in AECs of lung tissue from patients with IPF. (A) Representative results of HE staining and Masson staining in normal lung and lungs of patients with IPF (original magnification, 200×). (B) Representative images of dual staining for SPC (green) and P21 (red), FUT8 (green) and P21 (red), LCA (green) and P21 (red), and LCA (green) and FUT8 (red) (original magnification, 200×). (C) Western blotting was applied to detect the levels of activated P21, P16, and FUT8. (D) Lectin blot analysis of the immunoprecipitated IGFR-1 protein. IGFR-1 was immunoprecipitated from whole cell lysates using anti-IGFR-1 antibodies. The blots were probed with LCA. Representative data are shown. Quantification is shown in the lower panel. #P < 0.01 for the comparison between the control group and the IPF group. Unpaired, two-tailed Student’s t test.