Research Paper Volume 13, Issue 14 pp 18852—18869

Regulation of the IGF1 signaling pathway is involved in idiopathic pulmonary fibrosis induced by alveolar epithelial cell senescence and core fucosylation

CF was increased in AECs of lung tissue from patients with IPF. (A) Representative results of HE staining and Masson staining in normal lung and lungs of patients with IPF (original magnification, 200×). (B) Representative images of dual staining for SPC (green) and P21 (red), FUT8 (green) and P21 (red), LCA (green) and P21 (red), and LCA (green) and FUT8 (red) (original magnification, 200×). (C) Western blotting was applied to detect the levels of activated P21, P16, and FUT8. (D) Lectin blot analysis of the immunoprecipitated IGFR-1 protein. IGFR-1 was immunoprecipitated from whole cell lysates using anti-IGFR-1 antibodies. The blots were probed with LCA. Representative data are shown. Quantification is shown in the lower panel. #P

Figure 2. CF was increased in AECs of lung tissue from patients with IPF. (A) Representative results of HE staining and Masson staining in normal lung and lungs of patients with IPF (original magnification, 200×). (B) Representative images of dual staining for SPC (green) and P21 (red), FUT8 (green) and P21 (red), LCA (green) and P21 (red), and LCA (green) and FUT8 (red) (original magnification, 200×). (C) Western blotting was applied to detect the levels of activated P21, P16, and FUT8. (D) Lectin blot analysis of the immunoprecipitated IGFR-1 protein. IGFR-1 was immunoprecipitated from whole cell lysates using anti-IGFR-1 antibodies. The blots were probed with LCA. Representative data are shown. Quantification is shown in the lower panel. #P < 0.01 for the comparison between the control group and the IPF group. Unpaired, two-tailed Student’s t test.