Research Paper Volume 13, Issue 14 pp 18852—18869

Regulation of the IGF1 signaling pathway is involved in idiopathic pulmonary fibrosis induced by alveolar epithelial cell senescence and core fucosylation

CTGF, TGF1, and MMP9 were inhibited upon FUT8 knockdown in vitro and in vivo. (A) A549 and AECs were stimulated by IGF1 for 72 h, the supernatants were collected, and the levels of CTGF, TGF 1, and MMP9 were measured using ELISA. #P B) CTGF, TGF1, and MMP9 levels were assessed using western blotting analyses in vivo. *P

Figure 6. CTGF, TGF1, and MMP9 were inhibited upon FUT8 knockdown in vitro and in vivo. (A) A549 and AECs were stimulated by IGF1 for 72 h, the supernatants were collected, and the levels of CTGF, TGF 1, and MMP9 were measured using ELISA. #P < 0.01, ##P < 0.01. #Indicates the comparison of the control group with the IGF1 group; ## indicates the comparison of the FUT8siRNA+ IGF1 group with the IGF1 group. (B) CTGF, TGF1, and MMP9 levels were assessed using western blotting analyses in vivo. *P < 0.01. **P < 0.01. * indicates the comparison of the control group with BLM group; ** indicates the comparison of the FUT8shRNA+ BLM group with the BLM group. Data are shown as the mean ± SEM, n ≥ 3 per group. One-way ANOVA followed by Dunnett’s Multiple Comparison Test.