Research Paper Volume 13, Issue 14 pp 18852—18869

Regulation of the IGF1 signaling pathway is involved in idiopathic pulmonary fibrosis induced by alveolar epithelial cell senescence and core fucosylation

The fibroblast transition into myofibroblasts induced by the supernatants of senescent AECs can be inhibited by FUT8 knockdown. (A) After IGF1 was added to induce AEC senescence for 72 h, the medium was replaced by fresh medium without IGF1 to culture for another 3 days, then the supernatants were collected to culture MRC5 cells. The expression of α-SMA (green) and collagen I (red) were investigated using immunofluorescence (original magnification, 200×). (B) The expression of fibrotic markers, α-SMA and collagen I, were investigated by western blotting in vivo. (C) Representative images of HE and Masson’s trichrome-stained BLM lung sections (original magnification, 200×). #P P FUT8siRNA+ SASP-CM group with the SASP-CM group. *P P FUT8shRNA+BLM group with the BLM group. Data are shown as the mean ± SEM, n ≥ 3 per group. One-way ANOVA followed by Dunnett’s Multiple Comparison Test.

Figure 7. The fibroblast transition into myofibroblasts induced by the supernatants of senescent AECs can be inhibited by FUT8 knockdown. (A) After IGF1 was added to induce AEC senescence for 72 h, the medium was replaced by fresh medium without IGF1 to culture for another 3 days, then the supernatants were collected to culture MRC5 cells. The expression of α-SMA (green) and collagen I (red) were investigated using immunofluorescence (original magnification, 200×). (B) The expression of fibrotic markers, α-SMA and collagen I, were investigated by western blotting in vivo. (C) Representative images of HE and Masson’s trichrome-stained BLM lung sections (original magnification, 200×). #P < 0.01, ##P < 0.01. #Indicates the comparison of the control group with the SASP-CM group; ##indicates the comparison of the FUT8siRNA+ SASP-CM group with the SASP-CM group. *P < 0.01, **P < 0.01. * indicates the comparison of the control group with the BLM group; ** indicates the comparison of the FUT8shRNA+BLM group with the BLM group. Data are shown as the mean ± SEM, n ≥ 3 per group. One-way ANOVA followed by Dunnett’s Multiple Comparison Test.