Figure 8. CF regulates AEC senescence through the IGF1/PI3K/AKT pathways in vitro. (A) The IGFR-1 level in total cell lysates was assessed using western blotting analyses. Lectin blot analysis of the immunoprecipitated IGFR-1 protein. IGFR-1 was immunoprecipitated from whole cell lysates using anti-IGFR-1 antibodies. The blots were probed with LCA. Representative data are shown. Quantification is shown in the lower panel. (B) PI3K, AKT and p-AKT levels were assessed using western blotting analyses. Total cell lysates were subjected to immunoblotting. #P < 0.01, ##P < 0.01, # indicates the comparison of the control group with the IGF1 group; ## indicates the comparison of the FUT8siRNA+ IGF1 group with the IGF1 group. One-way ANOVA followed by Dunnett’s Multiple Comparison Test. Each experiment was performed in triplicate.