Research Paper Volume 13, Issue 14 pp 18852—18869

Regulation of the IGF1 signaling pathway is involved in idiopathic pulmonary fibrosis induced by alveolar epithelial cell senescence and core fucosylation

CF regulates AEC senescence through the IGF1/PI3K/AKT pathways in vitro. (A) The IGFR-1 level in total cell lysates was assessed using western blotting analyses. Lectin blot analysis of the immunoprecipitated IGFR-1 protein. IGFR-1 was immunoprecipitated from whole cell lysates using anti-IGFR-1 antibodies. The blots were probed with LCA. Representative data are shown. Quantification is shown in the lower panel. (B) PI3K, AKT and p-AKT levels were assessed using western blotting analyses. Total cell lysates were subjected to immunoblotting. #P ##P # indicates the comparison of the control group with the IGF1 group; ## indicates the comparison of the FUT8siRNA+ IGF1 group with the IGF1 group. One-way ANOVA followed by Dunnett’s Multiple Comparison Test. Each experiment was performed in triplicate.

Figure 8. CF regulates AEC senescence through the IGF1/PI3K/AKT pathways in vitro. (A) The IGFR-1 level in total cell lysates was assessed using western blotting analyses. Lectin blot analysis of the immunoprecipitated IGFR-1 protein. IGFR-1 was immunoprecipitated from whole cell lysates using anti-IGFR-1 antibodies. The blots were probed with LCA. Representative data are shown. Quantification is shown in the lower panel. (B) PI3K, AKT and p-AKT levels were assessed using western blotting analyses. Total cell lysates were subjected to immunoblotting. #P < 0.01, ##P < 0.01, # indicates the comparison of the control group with the IGF1 group; ## indicates the comparison of the FUT8siRNA+ IGF1 group with the IGF1 group. One-way ANOVA followed by Dunnett’s Multiple Comparison Test. Each experiment was performed in triplicate.