Research Paper Volume 13, Issue 16 pp 20149—20163

25-Hydroxycholesterol protecting from cerebral ischemia-reperfusion injury through the inhibition of STING activity

25-Hydroxysterol reduces size of cerebral infarction in a mouse middle cerebral artery occlusion (MCAO) model. Mice were randomly divided into three groups. Control group, healthy mice; MCAO group, mice with MCAO; mice with MCAO treated with + 25-Hydroxymethosterol. (A) Chemical structure of 25-Hydroxysterol. (B) Neurobehavioral score and Western blot analysis of BCL2 and Bax expression to select drug concentration (**P C) TTC staining was used to detect cerebral infarction in mice (N = 5 per group, **PP

Figure 1. 25-Hydroxysterol reduces size of cerebral infarction in a mouse middle cerebral artery occlusion (MCAO) model. Mice were randomly divided into three groups. Control group, healthy mice; MCAO group, mice with MCAO; mice with MCAO treated with + 25-Hydroxymethosterol. (A) Chemical structure of 25-Hydroxysterol. (B) Neurobehavioral score and Western blot analysis of BCL2 and Bax expression to select drug concentration (**P < 0.01 versus Sham group, #P <0.05 versus MCAO group, &P<0.05 versus MCAO+25-HC 0.1mg/kg group). (C) TTC staining was used to detect cerebral infarction in mice (N = 5 per group, **P<0.001 versus sham group, #P<0.05 versus MCAO group).