Research Paper Advance Articles

Myeloid DJ-1 deficiency protects acetaminophen-induced acute liver injury through decreasing inflammatory response

Myeloid DJ-1 deficiency attenuates APAP induced hepatic inflammatory reaction via less ROS production and chemokines induction. Fasted DJ-1fl/fl and Lysm-DJ-1−/− mice were intraperitoneal injected with a single dose of 300 mg/kg of APAP. Quantification of Ly6G+CD11b+ neutrophils (A) and Ly6C+CD11b+ macrophages (B) by flow cytometry (n = 4). (C) Quantification of CXCL1 and CXCL2 expression in liver tissue 24 h after APAP challenge (n = 5–8). (D) Quantification of cellular ROS levels by oxidized DCFDA and flow cytometry in neutrophils and macrophages isolated from DJ-1fl/fl and Lysm-DJ-1−/− mice (n = 4). Data are shown as the means ± SD, *P **P ***P ****P

Figure 6. Myeloid DJ-1 deficiency attenuates APAP induced hepatic inflammatory reaction via less ROS production and chemokines induction. Fasted DJ-1fl/fl and Lysm-DJ-1−/− mice were intraperitoneal injected with a single dose of 300 mg/kg of APAP. Quantification of Ly6G+CD11b+ neutrophils (A) and Ly6C+CD11b+ macrophages (B) by flow cytometry (n = 4). (C) Quantification of CXCL1 and CXCL2 expression in liver tissue 24 h after APAP challenge (n = 5–8). (D) Quantification of cellular ROS levels by oxidized DCFDA and flow cytometry in neutrophils and macrophages isolated from DJ-1fl/fl and Lysm-DJ-1−/− mice (n = 4). Data are shown as the means ± SD, *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.