Research Paper Volume 13, Issue 16 pp 20179—20191

Long non-coding RNA ABHD11-AS1 promotes colorectal cancer progression and invasion through targeting the integrin subunit alpha 5/focal adhesion kinase/phosphoinositide 3 kinase/Akt signaling pathway

ABHD11-AS1 deficiency attenuated proliferation and promoted apoptosis in CRC cells. (A) ABHD11-AS1 expression in HCT116-ABHD11-AS1-sh cells was determined by RT-qPCR. (B) ABHD11-AS1 expression in SW480-ABHD11-AS1-sh cells was determined by RT-qPCR. (C) The proliferation curves of HCT116-sh-NC cells and HCT116-ABHD11-AS1-sh cells measured by CCK-8 array. (D) The proliferation curves of SW480-sh-NC cells and SW480-ABHD11-AS1-sh cells measured by CCK-8 array. (E) PI staining illustrated the cell cycle distribution of HCT116-sh-NC cells and HCT116-ABHD11-AS1-sh cells. (F) PI staining illustrated the cell cycle distribution of SW480-sh-NC cells and SW480-ABHD11-AS1-sh cells. (G) Annexin V-7ADD and PI double staining illustrated the apoptosis cells of HCT116-sh-NC cells and HCT116-ABHD11-AS1-sh cells. (H) Annexin V-7ADD and PI double staining illustrated the apoptosis cells of SW480-sh-NC cells and SW480-ABHD11-AS1-sh cells. *P

Figure 2. ABHD11-AS1 deficiency attenuated proliferation and promoted apoptosis in CRC cells. (A) ABHD11-AS1 expression in HCT116-ABHD11-AS1-sh cells was determined by RT-qPCR. (B) ABHD11-AS1 expression in SW480-ABHD11-AS1-sh cells was determined by RT-qPCR. (C) The proliferation curves of HCT116-sh-NC cells and HCT116-ABHD11-AS1-sh cells measured by CCK-8 array. (D) The proliferation curves of SW480-sh-NC cells and SW480-ABHD11-AS1-sh cells measured by CCK-8 array. (E) PI staining illustrated the cell cycle distribution of HCT116-sh-NC cells and HCT116-ABHD11-AS1-sh cells. (F) PI staining illustrated the cell cycle distribution of SW480-sh-NC cells and SW480-ABHD11-AS1-sh cells. (G) Annexin V-7ADD and PI double staining illustrated the apoptosis cells of HCT116-sh-NC cells and HCT116-ABHD11-AS1-sh cells. (H) Annexin V-7ADD and PI double staining illustrated the apoptosis cells of SW480-sh-NC cells and SW480-ABHD11-AS1-sh cells. *P<0.05, **P<0.01, ***P<0.001.