Research Paper Volume 13, Issue 16 pp 20179—20191

Long non-coding RNA ABHD11-AS1 promotes colorectal cancer progression and invasion through targeting the integrin subunit alpha 5/focal adhesion kinase/phosphoinositide 3 kinase/Akt signaling pathway

ABHD11-AS1 deficiency inhibited ITGA5/Fak/PI3K/Akt signaling pathway in CRC. (A) mRNA sequencing to find differential genes between HCT116-sh-NC cells and ABHD11-AS1 deficient cells and displayed via Heat-map. (B) Differential genes enriched by KEGG pathway. Top 16 pathways were illustrated. (C) RT-qPCR to detect mRNA expression of ITGA5, CREB3L3, ITGA5, IL7 in HCT116 cells and ABHD11-AS1 deficient cells. (D) RT-qPCR to detect mRNA expression of ITGA5, CREB3L3, ITGA5, IL7 in SW480 cells and SW480 ABHD11-AS1 deficient cells. (E) WB analysis of protein abundance of ITGA5, FAK, p-FAK, P85, p-P85, Akt1, p-Akt1 in HCT116 cells and HCT116 ABHD11-AS1 deficient cells. (F) WB analysis of protein abundance of ITGA5, FAK, p-FAK, P85, p-P85, Akt1, p-Akt1 in SW480 cells and SW480 ABHD11-AS1 deficient cells. (G) WB analysis of protein abundance of ITGA5, FAK, p-FAK, P85, p-P85, Akt1, p-Akt1 in tumor tissues of nude mice. ***P

Figure 5. ABHD11-AS1 deficiency inhibited ITGA5/Fak/PI3K/Akt signaling pathway in CRC. (A) mRNA sequencing to find differential genes between HCT116-sh-NC cells and ABHD11-AS1 deficient cells and displayed via Heat-map. (B) Differential genes enriched by KEGG pathway. Top 16 pathways were illustrated. (C) RT-qPCR to detect mRNA expression of ITGA5, CREB3L3, ITGA5, IL7 in HCT116 cells and ABHD11-AS1 deficient cells. (D) RT-qPCR to detect mRNA expression of ITGA5, CREB3L3, ITGA5, IL7 in SW480 cells and SW480 ABHD11-AS1 deficient cells. (E) WB analysis of protein abundance of ITGA5, FAK, p-FAK, P85, p-P85, Akt1, p-Akt1 in HCT116 cells and HCT116 ABHD11-AS1 deficient cells. (F) WB analysis of protein abundance of ITGA5, FAK, p-FAK, P85, p-P85, Akt1, p-Akt1 in SW480 cells and SW480 ABHD11-AS1 deficient cells. (G) WB analysis of protein abundance of ITGA5, FAK, p-FAK, P85, p-P85, Akt1, p-Akt1 in tumor tissues of nude mice. ***P<0.001.