Figure 1. MiR-1252-5p expression in PAC cell lines and tissue samples. (A) qRT-PCR assay analyses of miR-1252-5p expression in five human PAC cell lines and HPNE. (B) qRT-PCR assay analysis of miR-1252-5p expression in 102 matched fresh human TT and NAT pancreas samples. Data were obtained using the 2-ΔΔCT method and were normalized to U6 levels. (C, D) In situ hybridization assay and staining score analyses were used to determine miR-1252-5p expression levels in 102 matched human TT and NAT pancreas samples. Scale bar, 200μm; scale bar for inserts, 40μm. (E) Kaplan-Meier survival curves showed association of miR-1252-5p expression with overall survival in patients with PAC (P = 0.034, log-rank test). PAC, pancreatic cancer; TT, tumor tissues; NAT, non-cancerous adjacent tissues. *, P<0.05; **P < 0.01; ***, P < 0.001.