Research Paper Volume 13, Issue 14 pp 18924—18945

MicroRNA-1252-5p, regulated by Myb, inhibits invasion and epithelial-mesenchymal transition of pancreatic cancer cells by targeting NEDD9

MiR-1252-5p inhibited tumor xenograft growth of PAC in vivo. (A, B) Tumor volumes and weights were represented. Scale bar, 1.0 cm. (C) The tumor sections were subjected to H&E staining and IHC staining using antibodies against ki-67. Scale bar, 100μm. (D) qRT-PCR assay analysis of the expression of miR-1252-5p in the xenograft tumors. (E, F) Western blot and IHC staining assay analysis of protein expression of NEDD9, E-cad, Vim, and ZEB1 in the xenograft tumors. Scale bar, 200μm. Data are presented as mean ± SD from triplicate experiments. IHC, immunohistochemistry. **P P

Figure 6. MiR-1252-5p inhibited tumor xenograft growth of PAC in vivo. (A, B) Tumor volumes and weights were represented. Scale bar, 1.0 cm. (C) The tumor sections were subjected to H&E staining and IHC staining using antibodies against ki-67. Scale bar, 100μm. (D) qRT-PCR assay analysis of the expression of miR-1252-5p in the xenograft tumors. (E, F) Western blot and IHC staining assay analysis of protein expression of NEDD9, E-cad, Vim, and ZEB1 in the xenograft tumors. Scale bar, 200μm. Data are presented as mean ± SD from triplicate experiments. IHC, immunohistochemistry. **P < 0.01; ***P < 0.001.