Figure 7. SRC/STAT3 signaling was essential for the biological function of miR-1252-5p/NEDD9 in PAC. (A, B) Western blot analysis of the activation of SRC/STAT3 signaling protein after transfection with corresponding vectors in PAC cells. C-H, MTT, wound healing and western blotting assays analysis of cell proliferation (C, D), migration (E, F) and EMT process (G, H) after transfection with corresponding vectors or treatment with PP1 in PAC cells. NEDD9, neural precursor cell expressed, developmentally downregulated 9; NC, negative control. E-cad, E-cadherin; N-cad, N-cadherin; Vim, Vimentin. ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001.