Research Paper Volume 13, Issue 14 pp 18924—18945

MicroRNA-1252-5p, regulated by Myb, inhibits invasion and epithelial-mesenchymal transition of pancreatic cancer cells by targeting NEDD9

MiR-1252-5p overexpression reversed Myb-induced cellular biological behaviors. (A, B) MTT assay analyses of cell proliferation in PAC cells after transfection with the indicated vectors. (C, D) Wound healing assay analyses of cell migration in PAC cells after transfection with the indicated vectors. (E) Western blot assay analyses of expression of NEDD9, EMT markers, SRC/STAT3 protein in PAC cells after transfection with the indicated vectors. (F) A working model depicts the mechanism of transcriptional regulation of Myb on miR-1252-5p targeting NEDD9 and the subsequent activation of SRC/STAT3 signal pathways contributed to PAC progression. E-cad, E-cadherin; Vim, Vimentin. Data are presented as mean ± SD from triplicate experiments. *, PP P

Figure 9. MiR-1252-5p overexpression reversed Myb-induced cellular biological behaviors. (A, B) MTT assay analyses of cell proliferation in PAC cells after transfection with the indicated vectors. (C, D) Wound healing assay analyses of cell migration in PAC cells after transfection with the indicated vectors. (E) Western blot assay analyses of expression of NEDD9, EMT markers, SRC/STAT3 protein in PAC cells after transfection with the indicated vectors. (F) A working model depicts the mechanism of transcriptional regulation of Myb on miR-1252-5p targeting NEDD9 and the subsequent activation of SRC/STAT3 signal pathways contributed to PAC progression. E-cad, E-cadherin; Vim, Vimentin. Data are presented as mean ± SD from triplicate experiments. *, P<0.05; **P < 0.01; ***, P < 0.001.