Research Paper Volume 13, Issue 15 pp 19415—19441

Development and validation of an intra-tumor heterogeneity-related signature to predict prognosis of bladder cancer: a study based on single-cell RNA-seq

Evaluation of ITH with MATH in BCa. (A) The workflow of this study. (B) The patients with high MATH values suffered an unfavorable prognosis. (C) The estimated MATH values acted as a potential predictor for chemosensitivity with Wilcoxon signer-rank test. (D) MATH values were positively correlated with TMB. (E) The cases in the high-MATH group had significantly lower immune and stromal components in TME. (F) Spearman correlation analysis indicated MATH values were negatively correlated with routine immune checkpoint genes, including PD1, LAG3, GAL9, CTLA4, TIM-3, and TIGIT. The red lines and green lines represented positive correlation and negative correlation, respectively. The boldness of the lines was positively associated with the strength of the correlation. *p **p ***p

Figure 1. Evaluation of ITH with MATH in BCa. (A) The workflow of this study. (B) The patients with high MATH values suffered an unfavorable prognosis. (C) The estimated MATH values acted as a potential predictor for chemosensitivity with Wilcoxon signer-rank test. (D) MATH values were positively correlated with TMB. (E) The cases in the high-MATH group had significantly lower immune and stromal components in TME. (F) Spearman correlation analysis indicated MATH values were negatively correlated with routine immune checkpoint genes, including PD1, LAG3, GAL9, CTLA4, TIM-3, and TIGIT. The red lines and green lines represented positive correlation and negative correlation, respectively. The boldness of the lines was positively associated with the strength of the correlation. *p < 0.05; **p < 0.01; ***p < 0.001.