Figure 5. Western blot analyses of the levels of (A, B) CD68 (C, D) ZO-1 in the control, 3-NP, and 3-NP+C16+Ang-1 groups revealed that treatment with C16+Ang-l can suppress 3-NP-induced inflammation, protect the blood vessels and promote neuroprotection. Detection of CD68 indicated microglia/macrophage activation. Zonula occludens protein-1 (ZO-1) is a marker protein for tight junctions in epithelial cells. ZO-1 protein is often destroyed in 3-NP-induced dystonia. Scale bar = 100 μm. aP < 0.05 versus control; bP < 0.05 versus 3-NP-treated mice.