Figure 9. (A–F) Treatment with C16+Ang-l can alleviate 3-NP-induced permeability of the blood-brain barrier (leakage of Evans Blue dye, red) and loss of tight junctions (ZO-1 immunostaining, green) in the (A, D) control, (B, E) 3-NP, and (C, F) 3-NP+C16+Ang-1 groups. Scale bar = 100 μm. (G, H) Semi-quantitative profiles of (G) Evans Blue staining and (H) ZO-1 immunostaining. aP < 0.05 versus control; bP < 0.05 versus 3-NP-treated mice.