Research Paper Volume 13, Issue 15 pp 19460—19474

Pedunculoside protects against LPS-induced mastitis in mice by inhibiting inflammation and maintaining the integrity of blood-milk barrier

Effect of PE on the integrity of blood-milk barrier in mice with mastitis in vivo. Fresh mice mammary gland was immersed in a 2 mg/mL FITC solution, quickly frozen in liquid nitrogen, and then frozen sections were prepared and obtained to evaluate the damage of the mice blood-milk barrier. Green fluorescence represents albumin fluorescein isothiocyanate conjugate protein province (FITC); blue represents nucleus. The arrows represent the leaking FITC in the damaged acinus and the shrinking acinar. The scale bar was 25 and 100 μM, respectively.

Figure 6. Effect of PE on the integrity of blood-milk barrier in mice with mastitis in vivo. Fresh mice mammary gland was immersed in a 2 mg/mL FITC solution, quickly frozen in liquid nitrogen, and then frozen sections were prepared and obtained to evaluate the damage of the mice blood-milk barrier. Green fluorescence represents albumin fluorescein isothiocyanate conjugate protein province (FITC); blue represents nucleus. The arrows represent the leaking FITC in the damaged acinus and the shrinking acinar. The scale bar was 25 and 100 μM, respectively.