Research Paper Volume 13, Issue 15 pp 19510—19528

UHPLC-MS-based metabolomics and chemoinformatics study reveals the neuroprotective effect and chemical characteristic in Parkinson’s disease mice after oral administration of Wen-Shen-Yang-Gan decoction

Immunofluorescence staining of TH protein in the substantia nigra of mice (A); Western blotting analysis (B) and quantification (C) of relative α-syn and TH protein abundance. Control: blank group; Model: rotenone-intoxicated group; WSYGD-H: high dosage group; WSYGD-M: medium dosage group; WSYGD-L: Low dosage group; Sinemet: positive control. **p

Figure 3. Immunofluorescence staining of TH protein in the substantia nigra of mice (A); Western blotting analysis (B) and quantification (C) of relative α-syn and TH protein abundance. Control: blank group; Model: rotenone-intoxicated group; WSYGD-H: high dosage group; WSYGD-M: medium dosage group; WSYGD-L: Low dosage group; Sinemet: positive control. **p<0.01,***p<0.001 vs control; #p<0.01, ##p<0.01, ###p<0.001 vs Model.