Figure 1. The expression landscape of circulating exosome-derived circRNAs in GBM patients. (A) Western blot was used to detect the expression of CD9, CD63, TSG101 and calnexin in exosome isolated from plasma samples of GBM patients (upper panel). Plasma exosomes were observed using TEM (lower panel). (B) Cluster analysis of the different expression of the circRNAs in different groups. (C) Volcano plot presented the dysregulated circRNAs in GBM comparing with control group. Red plots indicated with significant difference. (D) The expression of circRNAs were confirmed by RT-PCR in training set. * indicated P<0.05, n.s. indicated no significance.