Figure 3. Overexpression of AC136007.2 inhibits OGD/R-induced autophagy through inhibition of AMPK-mTOR signaling in SH-SY5Y cells. (A) Representative images of LC3 immunofluorescence and semi-quantitative analysis of autophagy levels (right). Cell nuclei were counterstained with DAPI. (B) Western blotting analysis of LC3 I/II, Beclin 1, p-AMPK, and p-mTOR expression and corresponding densitometric quantification data. Signals were normalized to β-actin. **p<0.01 versus control group; ## p<0.01 versus OGD/R group; ^^p<0.01 versus OGD/R + Lenti-AC136007.2 group; ^p<0.05 versus OGD/R + Lenti-AC136007.2 group; n = 3.